All products Antibodies Cytokeratin 19 [SA30-06]

Cytokeratin 19 [SA30-06]

Catalog Number：OM626618

  • Fig1: Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10,000 dilution.

  • Fig2: ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Fig7: Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Profile

Product Name Cytokeratin 19 [SA30-06]
Antibody Type Primary Antibodies
Product description 
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers.
Immunogen 
recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBICC/IFIHCFC
WB:1:5,000-1:10,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100
Species Reactivity

HumanMouse
Concentration 1 mg/mL.

Target Information

Alternative Names
40 kDa keratin intermediate filament antibody CK 19 antibody CK-19 antibody CK19 antibody Cytokeratin 19 antibody Cytokeratin-19 antibody K19 antibody K1C19_HUMAN antibody K1CS antibody Keratin 19 antibody Keratin type I 40 kD antibody Keratin type I 40kD antibody Keratin type I cytoskeletal 19 antibody Keratin
type I cytoskeletal 19 antibody Keratin
type I
40 kd antibody Keratin-19 antibody KRT19 antibody MGC15366 antibody
Molecular Weight(MW) 40 kDa
Cellular Localization Cytoplasm

Database Links

SwissProt ID P08727
P19001

Application

  • Application

    Fig1: Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10,000 dilution.

  • Application

    Fig2: ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.

  • Application

    Fig7: Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control Ags, MCF-7, human liver tissue, mouse liver tissue, human breast carcinoma tissue, human stomach carcinoma tissue.
Application Notes
WB:1:5,000-1:10,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:10-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM626618
  • Price: $386.00
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  • Lead time: 4~6 Week

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